Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope.

Three-dimensional imaging of living neurons and glia with the atomic force microscope.

The atomic force microscope (AFM) was used to directly image hippocampal neurons and glia. Using chemically fixed and living cells it was possible to reconstruct three-dimensional cell structure and detect sub-cellular features such as the nucleus, mitochondria and filaments. By repeatedly scanning a single living cell we observed the movement of filaments beneath the cell membrane. Furthermore...

Multimodal optical workstation for simultaneous linear, nonlinear microscopy and nanomanipulation: upgrading a commercial confocal inverted microscope.

In this work we propose and build a multimodal optical workstation that extends a commercially available confocal microscope (Nikon Confocal C1-Si) to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools such as nanosurgery. The setup allows both subsystems (confocal and nonlinear) to work independently and simultaneously. The workstation enables, for instance, na...

Intracellular nanosurgery and cell enucleation using a picosecond laser.

Living cells are highly organized in space and time, which makes spatially and temporally confined manipulations an indispensable tool in cell biology. Laser-based nanosurgery is an elegant method that allows precise ablation of intracellular structures. Here, we show cutting of fluorescently labelled microtubules and mitotic spindles in fission yeast, performed with a picosecond laser coupled ...

The Nonlinear Bending Analysis for Circular Nano Plates Based on Modified Coupled Stress and Three- Dimensional Elasticity Theories

In this paper, the nonlinear bending analysis for annular circular nano plates is conducted based on the modified coupled stress and three-dimensional elasticity theories. For this purpose, the equilibrium equations, considering nonlinear strain terms, are calculated using the least energy potential method and solved by the numerical semi-analytical polynomial method. According to the previous ...

Evaluation of Intrauterine Structural Pathology by Three-Dimensional Sonohysterography Using An Extended Imaging Method